Dr Mark Holcroft graduated with a Bachelor of Dental Surgery from the University of Adelaide in 1987. His early years as a new graduate  were conducted at the Clarendon Street Dental Practice. Mark diversified for a period, qualifying from Deakin University Victoria with a Master of Information Technology. In 2007 he rejoined the Practice to resume dentistry.

As well as being a practicing dentist Mark acts as a demonstrator to students  at LaTrobe University Dental School, Bendigo, Victoria.